回腸標(biāo)準(zhǔn)可消化組氨酸/賴氨酸對(duì)斷奶仔豬（7-11kg）生長(zhǎng)性能、腸道健康和含組氨酸蛋白動(dòng)員的影響

發(fā)布單位:天津瑞孚農(nóng)牧科技集團(tuán)有限公司

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時(shí)間：2023-02-17
      試驗(yàn)?zāi)康脑谟谠u(píng)估高于NRC需要量的回腸標(biāo)準(zhǔn)可消化組氨酸/賴氨酸（SID H/L）對(duì)斷奶仔豬（7-11kg）生長(zhǎng)性能、腸道健康和含組氨酸蛋白（包括血紅蛋白、肌肽和胰蛋白酶原）動(dòng)員的影響。
      試驗(yàn)選用40 頭斷奶仔豬（26日齡；初重7.1 ± 0.5 kg），根據(jù)性別和初始體重的完全隨機(jī)區(qū)組設(shè)計(jì)，分配至5種日糧處理。日糧中SID H/L分別為 26%、32%、38%、43% 和 49%，并飼喂14 天，日糧SID Lys含量為1.22%。在第0、7和 14 天分別記錄采食量和體重。第 12 天采集血樣。第14天對(duì)豬實(shí)施安樂(lè)死，采集胰腺、背最長(zhǎng)肌、空腸中段和空腸粘膜樣品。使用SAS的Proc Mixed對(duì)數(shù)據(jù)進(jìn)行統(tǒng)計(jì)分析。
      日糧處理對(duì)仔豬生長(zhǎng)性能沒(méi)有顯著影響，但是SID H/L顯著影響血紅蛋白水平（P<0.05，36%時(shí)濃度最高，為12 g/dL）、空腸粘膜中免疫球蛋白A水平（IgA，P<0.05，35%時(shí)濃度最低，為1.25 μg/mg），空腸中絨毛高度（P=0.065，40%時(shí)最大，為536 μm）、胰腺中胰蛋白酶原含量（P=0.083，41%時(shí)含量最高，為242 pg/mg）和肌肉中肌肽水平（P=0.051，38%時(shí)濃度最高，為4.7 ng/mg）。日糧SID H/L線性提高肌肉中的蛋白質(zhì)羰基（P<0.05，從1.95到2.80 nmol/mg），并減少腸細(xì)胞增殖（P<0.05，從29.1%到26.9%）。
      總之，回腸標(biāo)準(zhǔn)可消化組氨酸/賴氨酸在35%-41%之間可以改善仔豬（7-11kg）腸道健康，并使含組氨酸蛋白水平達(dá)到峰值，這表明含組氨酸的蛋白質(zhì)是確定組氨酸需要量時(shí)的有效標(biāo)準(zhǔn)。

The effects of standardized ileal digestible His to Lys ratio on growth performance, intestinal health, and mobilization of histidine-containing proteins in pigs at 7 to 11 kg body weight
The objectives were to evaluate the effects of standardized ileal digestible (SID) His:Lys ratio above the current NRC requirement on growth performance, intestinal health, and mobilization of His-containing proteins, including hemoglobin, carnosine, and trypsinogen, in nursery pigs from 7 to 11 kg body weight (BW). Forty pigs (26 d of age; initial BW of 7.1?±?0.5 kg) were allotted to 5 dietary treatments based on a randomized complete block design with sex and initial BW as blocks. Dietary treatments were supplemented with varying SID His to Lys ratios of 26%, 32%, 38%, 43%, and 49% and fed to pigs for 14 d (SID Lys?=?1.22%). Feed intake and BW were recorded at d 0, 7, and 14 to measure growth performance. Blood samples were collected on d 12. Pigs were euthanized on d 14 to collect pancreas, longissimus dorsi muscles, mid-jejunum, and jejunal mucosa. Data were analyzed using the Proc Mixed of SAS. Growth performance was not affected, whereas varying SID His to Lys ratio affected hemoglobin (P?<?0.05, max: 12 g/dL at 36%), immunoglobulin A (IgA, P?<?0.05, min: 1.25 μg/mg at 35%) in jejunal mucosa, villus height (P?=?0.065, max: 536 μm at 40%) in jejunum, trypsinogen (P?=?0.083, max: 242 pg/mg at 41%) in pancreas, and carnosine (P?=?0.051, max: 4.7 ng/mg at 38%) in muscles. Varying SID His to Lys ratios linearly increased (P?<?0.05, from 1.95 to 2.80 nmol/mg) protein carbonyl in muscles and decreased (P?<?0.05, from 29.1% to 26.9%) enterocyte proliferation. In conclusion, SID His to Lys ratio between 35% and 41% in diets fed to nursery pigs at 7 to 11 kg enhanced intestinal health and maximized concentrations of His-containing proteins, indicating that His-containing proteins are effective response criteria when determining His requirement.

文章來(lái)源：豬營(yíng)養(yǎng)國(guó)際論壇
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